Figure 4.

Cytokines and chemokines released into the supernatants of IL-17-stimulated RACs attract more inflammatory cells than those produced by IL-17-stimulated RPE cells. Spleen cells from IRBP1–20-immunized B6 mice that migrated to the lower chamber containing supernatants collected from RACs (A) or RPE cells (B) pretreated for 24 h with medium, IL-17 (100 ng/ml), IFN-γ (500 U/ml), or IL-17 and IFN-γ were counted after 2 h, stained with rat mAb against mouse TCR or Gr-1, and analyzed by flow cytometry. The results are representative of those obtained in three experiments. The values shown are the means ± sd. *, P < 0.05; **, P < 0.01.