Figure 2.
Combination of cell concentrations of T.b.brucei and reagent volumes for signal detection when a 384-well plate inoculated directly from flask culture dilutions and detected by the luciferase ATP detection assay. Fifty-five μL of T.b.brucei cell densities ranging from 1-3 × 106 cells/mL were transferred from a flask culture in to a 384 well plate and detected with CellTiter-Glo. Twenty-five, 20, 15 and 10 μL volumes of CellTiter-Glo were compared for signal detection and reproducibility.