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Signal window and % CV of samples from varying inocula of trypanosomes following 72 hours growth and detection with the luciferase viability assay. The assay signal window and % CV after 72 hours growth with differing trypanosome inocula were compared with both 15 μL (A) and 10 μL (B) of detectant. The signal window was calculated from media without a cell addition.
