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DMSO dose response curve for 500 cells/mL inoculum; and the Z' of pre-diluted DMSO at 0.417% and 0.208% final concentration in the assay for a 500 and 250 cells/mL inoculum density. (A) DMSO standard curve from 0 to 8.3% DMSO in the luciferase assay for a 500 cell/mL inoculum (B) DMSO concentration and Z' of 500 and 250 mL cell inocula with a pre-dilution of DMSO to decrease the assay concentration. The Z' was estimated from a quarter of a 384 well plate.
