Table 2.
Reference compound IC50's from reference compounds diluted in water, HMI-9 medium with and without FCS and PBS.
Compound | PBS | Water | FCS | No FCS |
---|---|---|---|---|
Pentamidine | 13.42 ± 7.5 | 57.78 ± 40 | 9.23 ± 0.71 | 7.56 ± 2.8 |
Diminazine | 65.98 ± 1.7 | 140.6 ± 79 | 65.79 ± 21 | 62.46 ± 21.3 |
Staurosporine | 10.99 ± 1.3 | 12.27 ± 3.0 | 2.43 ± 0.59 | 5.22 ± 1.1 |
IC50 values of compounds in variations of compound dilution media were determined in the luciferase assay and calculated from duplicate samples, expressed as ± SD, in nM.