Table 1.
Percentage (mean ± SD) of boar sperm cells with intact, detaching but still adherent, or completely detached head membranes after incubation with detergents for 30 min at 4°C or 38°C
| intact membranes | detaching membranes | detached membranes | ||||
|---|---|---|---|---|---|---|
| Detergent | 4°C | 38°C | 4°C | 38°C | 4°C | 38°C |
| w/o | 91,2 ± 8,5 | 92,7 ± 5,1 | 8,3 ± 8,6 | 6,1 ± 3,7 | 0,4 ± 0,7 | 1,2 ± 2,3 |
| Pluronic F-127 | 94,1 ± 4,5 | 95,3 ± 4,0 | 5,6 ± 4,7 | 4,1 ± 3,8 | 0,3 ± 0,5 | 1,0 ± 1,2 |
| Sodium cholate | 0,3 ± 0,8 | 0,1 ± 0,4 | 96,0 ± 3,4 | 97,1 ± 2,3 | 3,7 ± 3,1 | 2,7 ± 2,2 |
| CHAPS | 0,3 ± 0,8 | 0,0 ± 0,0 | 96,4 ± 5,7 | 94,7 ± 3,5 | 3,3 ± 5,8 | 5,3 ± 3,5 |
| Tween 20 | 43,1 ± 25,1** | 0,0 ± 0,0** | 56,0 ± 24,7** | 98,0 ± 1,7** | 0,9 ± 0,9 | 2,0 ± 1,7 |
| Triton X-100 | 0,1 ± 0,3 | 0,6 ± 0,9 | 97,2 ± 2,5 | 96,7 ± 2,0 | 2,7 ± 2,6 | 2,8 ± 1,9 |
| Brij 96V | 0,1 ± 0,3 | 0,3 ± 0,5 | 98,8 ± 1,9* | 91,8 ± 7,6* | 1,1 ± 1,6* | 7,9 ± 7,7* |
* significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,05, n = 9)
** significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,01, n = 9)
Sperm cells of ejaculates from 9 different boars were treated and fixed as described in the "Materials and Methods" section. 200 cells per sample were counted under phase contrast optics (magnification of 1000).