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. 2009 Oct 29;2:219. doi: 10.1186/1756-0500-2-219

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Copyright ©2009 Mitsouras et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amplification of an 1.1-kb fragment using DNA isolated from saliva, buccal swab and blood samples. 100 ng of canine genomic DNA isolated using the Oragene ^®•ANIMAL kit (An; lanes 1, 4, 7, 9, 11, 13, 15, 17, 19, 21, 24, 26, 28, 30 and 32), buccal swabs (Bu; lanes 2, 5, 8, 10, 12, 14, 16, 18, 20, 22, 25, 27, 29, 31 and 33) or blood (Bl; lanes 3, 6 and 23) were used for PCR amplification of an 1155-bp fragment in the coding region of the canine transferrin receptor gene. Reaction products were resolved by agarose gel electrophoresis and visualized by staining with SYBR^®Green.