Fig. 7.

Defects in tubulogenesis in LanB1 mutants. (A-B′) Embryos labelled with anti-DE-cadherin (DE-cad, red) and anti-GFP (green) antibodies to visualise the proventricular cells and to mark the balancer chromosome, respectively. (A′,B′) Magnifications of the proventriculus shown in A and B. (A,A′) In wild-type embryos, proventricular cells have moved inward by stage 16 (arrow in A′). (B,B′) This movement fails in homozygous LanB1^1B1 embryos (arrow in B′). (C,D) Second instar larvae that have been fed with yeast containing red dye. Whereas wild-type larvae can feed normally and show a coloured gut (C), homozygous LanB1^28a larvae do not (D). (E) Wild-type Malpighian tubules of stage 17 embryos consist of four long tubes, as seen with a principal cell marker, anti-Cut antibody (green). (F) LanB1^1B1 mutant Malpighian tubules are short and fat, but like the wild type contain principal (green) and stellate (red) cells (E,F, white boxes).