Fig. 3.

Notch intracellular domain and TTK69 expression during late oogenesis. (A-G) Confocal projections of wild-type egg chambers stained for N intracellular domain (N-ICD) and TTK69 collected using uniform exposure conditions. (A) High N and low TTK69 in S6-S9. (B) N clears to the T at early S10B and TTK69 is elevated. (C) No N expression remains in follicle cells at S11 except for a small region in the posterior (not shown). TTK69 levels become higher in the anterior. (D) At S13, N remains off and TTK69 is expressed yet more strongly in the anterior. At all stages, N is expressed in the nurse cells. (E-G) Three examples of early S10B egg chambers showing variable TTK69 expression. Lines indicate approximate dorsal midline position. (H-H″) A ttk^twk clone at early S10B (T stage). At this stage, TTK69 is reduced but not eliminated in ttk^twk cells. Scale bars: 20 μm.