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. 2009 Dec 1;2(4):300–309. doi: 10.1593/tlo.09172

Figure 3.

Figure 3

Overexpression of SWAP-70 induces changes in cell morphology. (A) U87 cells were selected for SWAP-70 overexpression given the relatively low endogenous levels of SWAP-70 and transient transfection with pFLAG-SWAP-70. (Single band in pFLAG lane is nonspecific cross-reacting protein that runs at a slightly lower molecular weight than SWAP-70.) (B) SWAP-70 was mainly localized to the perinuclear cytoplasm and actin-rich membrane ruffles at the leading edge (arrow) in serum-starved pFLAG-U87 cells. pFLAG-SWAP-70 cells showed slightly increased membrane ruffles and redistribution of SWAP-70, even in serum-starved condition, and cells appeared to have increased projections giving the cells a stellate appearance. Scale bar, 10 µm. SW70 indicates SWAP-70.