Skip to main content
. 2009 Dec 1;2(4):300–309. doi: 10.1593/tlo.09172

Figure 6.

Figure 6

Effects of SWAP-70 expression on glioma cell migration and invasion. Radial cell migration assays were performed on (A) U87 glioma cells overexpressing SWAP-70 or (B) T98 and SNB19 glioma cells transfected with SWAP-70 siRNA. Cells were transfected, then 48 hours after transfection, the cells were seeded and allowed to migrate for 24 hours. Images were acquired before and after migration, and the average migration rate was calculated during 24 hours. *P < .05, significant changes in migration after transfection. (C) Matrigel cell invasion assay in transient overexpressing and gene-silenced glioma cell lines. A modified Boyden chamber with Matrigel-precoated membrane filter insert was used to measure in vitro invasiveness. At 24 hours after seeding the cells, the invading cells were fixed and stained. Representative images are shown (left panels). Original magnification, x200. Invasive cells were counted in six random fields. Invasiveness was increased in U87 cells overexpressing SWAP-70. In contrast, the invasiveness was decreased in both T98 and SNB19 glioma cells in which SWAP-70 protein levels had been knocked down by RNA interference. *P < .05, significant changes in invasion after modulation of SWAP-70 expression levels.