Skip to main content
. 2009 Dec 1;2(4):321–328. doi: 10.1593/tlo.09193

Figure 1.

Figure 1

Sorting of anti-CD44 antibodies-tagged untreated (control) and FOLFOX-resistant HCT-116 cells by flow cytometry. FOLFOX-resistant HCT-116 cells were generated by exposing HCT-116 to 25 µM 5-FU and 0.625 µM oxaliplatin for 48 to 72 hours and subsequently cultured them in normal medium without the drugs for 4 to 5 days. The cycle was repeated 12 times. The surviving cells were then split and exposed to higher doses of FOLFOX (50 µM 5-FU + 1.25 µM oxaliplatin or 100 µM 5-FU + 2.5 µM oxaliplatin) for 2 to 3 days a week for approximately 4 weeks. The FOLFOX-surviving cells were maintained in normal culture medium containing a low dose of FOLFOX (5 µM 5-FU + 0.125 µM oxaliplatin).