Figure 1.
Effect of Ca-recoverin on the flash response of a single truncated outer segment. Plots of membrane current as a function of time. Sequential responses to flashes (80 photons/μm2, delivered at times indicated by arrows) during dialysis with 0.5 μM Ca (A), 20 μM Ca (B), 20 μM Ca with 8.6 μM recoverin (C), 0.5 μM Ca with 8.6 μM recoverin (D), and 0.5 μM Ca only (E). Recovery phases were fitted by single exponentials (dashed lines) with time constants, τ, of (s): 6.0 (A and B); 21 (C); 8.8 (D); 11 (E). Response prolongation, defined as τhigh Ca-rec/τcontrol, was 3.5. The recoverin was myristoylated.