Skip to main content
. 2009 Dec 7;4(12):e8202. doi: 10.1371/journal.pone.0008202

Figure 1. CpdA mediates transrepression in myelin-specific T effector cells via the GR but not transactivation.

Figure 1

(A) Tenc cells were cultured in the presence of APCs and 10 µg/ml gpMBP, either treated with 10−6 M Dex or 10−5 M CpdA for 6 hrs or left untreated (con). IFNγ and IL-17 production was assessed by intracellular flow cytometry and normalized to each control (100%) based on the percentage of cells positively staining for the respective cytokines. n = 3. (B) Tenc cells were cultured in the presence of 10−6 M Dex or 10−5 M CpdA for 6 hrs followed by the analysis of GILZ mRNA expression by quantitative RT-PCR. The expression levels were normalized to the housekeeping gene β-actin and the results are depicted as fold induction relative to untreated control cells. n = 3. (C) MEFs derived from GRN+/+ mice (wildtype cells) and GRN−/− mice (GR-deficient cells) were preincubated with 5 ng/ml PMA for 1 hr. Subsequently, they were stimulated with 10−6 M Dex or 10−5 M CpdA for 5 hrs or left untreated (con). MMP-13 mRNA expression was determined by quantitative RT-PCR and normalized to β-actin. MMP-13 levels in the medium controls were set as 100%. n = 3. *: p<0.05, **: p<0.01, ***: p<0.001, n.s.: p>0.05.