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. 2009 Nov 18;6(6):338–347. doi: 10.7150/ijms.6.338

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The figure shows the cell cycle distribution of DU 145 cells: In the left part of the figure the plot of the untreated control is demonstrated, the middle and the right plot show the cell cycle distribution 144 h after treatment with TMZ-BioShuttle and TMZ respectively. The prostate cancer cell line exhibits two cell fractions: a diploid (DNA-index of 1.0)[red coloured] and an aneuploid fraction (DNA-index 1.1)[blue coloured], close to the diploid. The G1 and the G2M peaks show a diploid and an aneuploid DNA content respectively. S-phase: After TMZ treatment (right plot) the cell number of the aneuploid cells is 10% higher (59 %) compared to aneuploid S-Phase cells in the TMZ-BioShuttle treated probe (middle plot). This in turn was 1.8 fold increased (49 %) compared to the corresponding cell fraction of the control (27 %). The relative amount of the diploid cell fraction differs from the aneuploid fraction: The control shows 35 % diploid cells. The aneuploid fractions reveal decreased amounts 15% (TMZ-BioShuttle) and 3% (TMZ). G2/M phase: The cell cycle behaviour of both cell fractions, the diploid and the aneuploid, show partly opposing effects. In comparison to the control which shows identical percentage of the diploid and the aneuploid cells, the TMZ and the TMZ-BioShuttle treated probes display an increased diploid cell fraction in which the TMZ-BioShuttle probe shows the highest cell contingent. The fraction of aneuploid cells is reduced to 11% whereas the diploid part is increased to 28% in the TMZ-BioShuttle treated probe. The amount of cells in the G2/M phase is increased in a similar ratio of 25 % diploid and 24 % aneuploid cells in the TMZ probe. G1-phase: The comparison of the amount of aneuploid and diploid cells in the G1 phase shows a ratio of 49 % and 54%. The cells in the G1 phase of the TMZ treated probe was increased to 73 % (diploid) and 57 % in the TMZ-BioShuttle probe. The aneuploid cell fractions exhibit an opposite result: the aneuploid cell fraction is decreased to 41% (TMZ-BioShuttle) and 17 % (TMZ).