Figure 2.

QIAxcel gel image CAV3 assays. Columns 1 through 11 contain PCR products from control DNA for CAV3 assays. The 15 base pair and 3000 base pair bands are reference markers injected before the PCR products are injected. Note that the 46XY control DNA that was used may be heterozygous for assay cv 08. Single bands and good forward sequence for this assay in several patients' DNA has been seen. Also there is a large CA repeat in the area which renders the reverse sequence poor. See Additional file 8 for size, concentration and gene coverage details.