Fig. 2.

DHA induces Foxp3, CTLA-4, TGF-β, and/or IL-10 mRNA expression. The cells were purified from the spleen of mice and preincubated without or with DHA (100 µM) for 4 h, prior to the total RNA extraction from Treg (A) and Teff cells (B). The mRNA expression of the genes was quantitatively determined by real-time PCR as described in Materials and Methods. Insets of A and B show the expression of Foxp3 protein in Treg and Teff cells, respectively, treated or not with DHA (100 μM).