Fig. 3.

DHA-treated Treg-like cells do not behave as true Treg cells. Teff cells as responder cells and autologous Treg-like cells (DHA-treated Teff cells, DHA at 100 µM) or Treg cells were cocultured with soluble anti-CD3 antibody for 46 h (A). Treg or Teff cells, containing or not DHA (100 μM), were stimulated with anti-CD3 (2 µg/ml) and anti-CD28 (5 µg/ml) antibodies for 46 h with or without IL-2 at 20 IU/ml (B). Cell proliferation was measured by [³H]thymidine incorporation. Results are expressed as mean ± SEM of experiments reproduced at least three times and performed in six replicates from different mice. **P < 0.001: sig­nificant difference compared with controls. NS, insignificant differences.