Fig. 7.
DHA modulates p27KIP1 mRNA expression in Treg and Teff cells. The cells were purified from the spleen of mice and incubated without or with DHA (100 μM) for 4 h prior to the total RNA extraction from Teff (A) and Treg (B) cells. The p27KIP1 mRNA expression was quantitatively determined by real-time PCR as described in Materials and Methods. *P < 0.05 and **P < 0.01: significant differences between DHA-treated and untreated cells. Inset shows the p27KIP1 protein in Western blots before and after DHA treatment of Treg and Teff cells. The blots used for p27KIP1 were stripped off and reprobed for β -actin.