Fig. 5.

Compound C affects the level of sphingolipids in MCF7 cells. GFP-Bax stable MCF7 cells were treated with 20 µM Compound C for 24 h. The extracted lipids from collected cells were subjected to HPLC and tandem MS analyses to determine sphingosine (A) and sphingomyelin (B) levels. Treatment with Compound C lowered the level of sphingosine (from 108.3 ± 9.9 to 36.3 ± 4.8 pmol/µmol Pi), but there was no significant change in the level of total sphingomyelin (17744.7 ± 876.5 pmol/µmol Pi for untreated versus 17478.5 ± 947.2 pmol/µmol Pi for treated). The results are expressed as a percentage of the control untreated cells. Data are means ± SEM. * P < 0.05, n = 3.