TABLE 1.

Compound C elevates the level of ceramide in MCF7 cells

	Con	20 µM CC	40 µM CC
17C14:0	248 ± 5	228 ± 8	306 ± 17
17C16:0	6,100 ± 563	25,600 ± 1,890*	34,300 ± 2,770*
17C18:0	707 ± 71	2,270 ± 200*	3,040 ± 247*
17C18:1	72 ± 2	74 ± 7	99 ± 8
17C20:0	491 ± 22	485 ± 47	550 ± 54
17C20:1	231 ± 4	281 ± 15	277 ± 24
17C22:0	285 ± 20	213 ± 17	285 ± 24
17C22:1	838 ± 18	870 ± 63	965 ± 6
17C24:0	445 ± 48	400 ± 42	536 ± 49
17C24:1	1,590 ± 161	1,660 ± 90	1,930 ± 155
17C26:0	331 ± 30	360 ± 28	382 ± 37
17C26:1	1,090 ± 62	1,230 ± 116	1,350 ± 119
Total	12,400 ± 445	33,700 ± 2,400*	44,100 ± 3,260*

GFP-Bax stable MCF7 cells were treated with 20 or 40 µM Compound C for 24 h. The extracted lipids from collected cells were subjected to HPLC and tandem MS analyses to determine total ceramide and ceramide species content (pmol/µmol Pi). Values are means ± SEM; n = 3. *P < 0.05.