TABLE 2.

Compound C triggers the upregulation of ceramide synthase activity in MCF7 cells

	Con	20 µM CC	40 µM CC
C14:0	18 ± 2	17 ± 2	20 ± 3
C16:0	774 ± 53	1,270 ± 112*	1,910 ± 107*
C18:0	4 ± 0.5	22 ± 0.8*	34 ± 3*
C18:1	19 ± 1	19 ± 2	23 ± 2
C20:0	9 ± 0.6	10 ± 0.8	13 ± 1
C20:1	2 ± 0.1	2 ± 0.1	2 ± 0.2
C22:0	24 ± 2	23 ± 3	25 ± 3
C22:1	12 ± 0.7	12 ± 1	14 ± 0.5
C24:0	298 ± 28	287 ± 21	325 ± 31
C24:1	223 ± 1	307 ± 22	448 ± 34*
C26:0	7 ± 0.3	7 ± 0.6	8 ± 0.9
C26:1	20 ± 0.7	21 ± 3	24 ± 3
Total	1,410 ± 66	1,990 ± 94*	2,840 ± 143*

GFP-Bax stable MCF7 cells were treated with 20 or 40 µM Compound C (CC) for 24 h. The cells were then labeled with 1 µM 17C-sphingosine, and ceramide synthase activities (pmol 17C-ceramide/µmol phosphate) of the collected cells were determined by tandem MS. Values are means ± SEM; n = 3. *P < 0.05.