Fig. 7.
Straight-phase HPLC on Lichrosorb Diol of a so-called steroid sulfate fraction from rat brain using the unreliable C18 SPE procedure. Each HPLC fraction was hydrolyzed and derivatized with HFBA at 20°C. The release of PREG (r-PREG) and DHEA (r-DHEA) (A) coincides with the elution of CHOL (B). The elution of 3H-PREGS in the same system is shown for comparison.