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. 2009 Dec;50(12):2430–2444. doi: 10.1194/jlr.M900162-JLR200

Fig. 7.

Fig. 7.

Straight-phase HPLC on Lichrosorb Diol of a so-called steroid sulfate fraction from rat brain using the unreliable C18 SPE procedure. Each HPLC fraction was hydrolyzed and derivatized with HFBA at 20°C. The release of PREG (r-PREG) and DHEA (r-DHEA) (A) coincides with the elution of CHOL (B). The elution of 3H-PREGS in the same system is shown for comparison.