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Epithelial Na channel (ENaC) β-subunit (β-ENaC) protein in total and surface membrane fractions of the kidney of rats fed control-, low-, or high-Na diet. Each lane represents a different animal. Lanes were loaded with 60 μg of total membrane protein or NeutrAvidin eluate from 600 μg of total membrane protein. Densities of bands were normalized to those of control samples. *P < 0.05 (experimental > control).
