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Exercise increases the levels of D1 receptor mRNAs and proteins. A: D1 receptor mRNA was amplified using gene specific primers and resolved on 0.8% agarose gel. The bands were normalized by glyceraldehydes-3-phosphate dehydrogenase. B: 20 μg RPT membrane protein was resolved by SDS-PAGE, and D1 receptor proteins were determined by immunoblotting with specific antibody; n = 4 animals/group. Results are means ± SD. P < 0.05 vs. AS (*) and vs. OS (#).
