Fig. 7.

A and B: effect of PKC isoenzyme-specific inhibitors on oxalate-induced membrane translocation of PKC-α (A) and PKC-δ (B) protein expression in LLC-PK₁ cells. LLC-PK₁ cells were pretreated with inhibitor peptide (2.5 μg/ml) or rottlerin (7.5 μM) for 30 min and then exposed to 0.75 mM oxalate along with inhibitors for 3 h. Lysates of the membrane fractions were analyzed for PKC-α (A) and PKC-δ (B) expression by Western blotting. DMSO was used as a vehicle. Data are normalized to control, and values are expressed as means ± SE. A typical blot from 1 of 3 experiments is shown. The graph represents the ratio of densitometric analysis of PKC-α or PKC-δ protein expression to Na⁺-K⁺-ATPase. Comparisons shown: a, significant compared with vehicle-treated control; b, significant compared with oxalate-treated cells; c, significant compared with inhibitor peptide- or rottlerin-treated cells. *P < 0.05; n = 3.