Fig. 5.

Effects of AKT and STAT3 inhibition on cell survival following H₂O₂ expsosure. A: RPTC were incubated with LY294002 (20 μM) or S3I201 (50 μM) for 1 h and then exposed to 1 mM H₂O₂ for 4 h (A) or 30 min (B). Cell viability was determined by MTT assay. Values are means ± SD of 3 independent experiments conducted in triplicates and expressed as percentage of control. Bars with different letters are significantly different from one another (P < 0.05; A). Cell lysates were subjected to immunoblot with antibodies for p-Ser473-AKT, AKT, p-Tyr705-STAT3, STAT3, or actin. Representative immunoblots from 3 experiments are shown in B.