Fig. 3.

Mean (+ SE) number of Kiss1 cells or Kiss1 mRNA per cell in the ARC of prepubertal females (A) and males (B) that were GDX or left intact on PND 14 and then euthanized 2 or 4 days later (i.e., on PND 16 or PND 18, respectively). Kiss1 gene expression in the ARC was significantly higher in GDX than in intact females on PND 16. In contrast, there was no significant difference in ARC Kiss1 expression between intact and GDX juvenile males. *Significantly different from intact animal of same age (P < 0.05).