Fig. 4.

Sex differences in the mean number of Kiss1 cells in the ARC (A) or anteroventral periventricular nucleus/periventicular nucleus continuum (AVPV/PeN; B) of prepubertal males and females that were GDX or left intact on PND 14 and euthanized 4 days later on PND 18. Brain tissue from animals of each sex was assayed together to directly assess sex differences in Kiss1 expression. Bars with different letters differ significantly from each other (P < 0.05). A direct comparison between GDX and intact males revealed a small but significant difference in the number of AVPV/PeN Kiss1 cells (P < 0.05). C: representative photomicrographs of Kiss1 neurons in the AVPV/PeN of prepubertal females and males that were left intact on PND 14 and then euthanized 4 days later (PND 18).