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. 2009 Sep 4;297(5):H1711–H1719. doi: 10.1152/ajpheart.00553.2009

Fig. 2.

Fig. 2.

Normoxic cardiac myocytes were infected with adenovirus carrying the green fluorescent protein (AdGFP), rat OGT (AdOGT), or rat O-GlcNAcase (AdGCA) gene and subjected to ER stress [with brefeldin A (BfA) or tunicamycin (TM)]. Whole cell lysates were immunoblotted for O-GlcNAc. A: OGT overexpression significantly augmented O-GlcNAc levels over those induced by BfA as shown in immunoblot and densitometric quantification of immunoblots. The BfA-induced increase in O-GlcNAc levels in GCA-overexpressed cells was less than baseline O-GlcNAc levels. B: OGT overexpression significantly augmented O-GlcNAc levels over those induced by TM. The TM-induced increase in O-GlcNAc levels in GCA-overexpressed cells did not reach baseline O-GlcNAc levels. *P < 0.05 vs. AdGFP; **P < 0.05 vs. AdGFP + BfA or AdGFP + TM; n ≥ 5/group.