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Immunocytochemistry for nNOS and estrogen receptors (ER). Colocalization of nNOS and ER in the SFO and PVN is shown in intact, untreated female mice. Representative sections show ER (red), nNOS (green), and double labeling (yellow) in SFO and PVN neurons. Magnification is the same for the top 2 rows; bottom row is at higher magnification of the sector indicated by the boxed area.
