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Intrinsic fluorescence of the recombinant TSRs. A, emission spectra of the recombinant TSRs at 25 °C. The proteins, 0.25 mg/ml, were excited with 295 nm. B, emission spectrums of P123 at different temperatures. C, changes in the peak emission (λmax) upon heating for the four constructs. Error bars, mean ± S.E. of at least three experiments.
