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Intrinsic fluorescence of C, CP123, P123, and a mixture of C and P123. A, emission spectrum of C, P123, CP123, and an equal molar mixture of C and P123 (C + P123), 0.25 mg/ml, excited at 295 nm at 25 °C. Both CP123 and C + P123 have their λmax at 343 nm. B, shift of λmax as a function of temperature. Error bars, mean ± S.E. of at least three experiments.
