FIGURE 5.

Effects of VDAC1 overexpression on the PQ-dependent H₂O₂ production and the cytotoxicity in HeLa cells. A, lysates from VDAC1-overexpressing cells were subjected to Western blot analysis with anti-VDAC1 mAb. Control, cells transfected with empty vector; VDAC+, cells transfected with the vector bearing vdac1 cDNA. B, H₂O₂ production by PQ in VDAC1-overexpressing cells (VDAC1+) was higher than that of control cells (*, p < 0.001). Light bars, no treatment; dark bars, exposure to 1 mm PQ. Error bars represent S.D. (n = 3). C, the survival rates of VDAC1-overexpressing HeLa cells (closed circle) were lower than those of controls (open circle; p < 0.001). Error bars represent S.E. of triplicate experiments.