TABLE 1.

Oxygen consumption kinetic parameters for the WT and Erv1p mutants

All of the experiments were carried out with 1 mm Erv1p proteins in 50 mm Tris buffer, pH 7.4, 150 mm NaCl, 1 mm EDTA at 25 °C. The error bars represent the standard errors (n = 3).

Electron donor	Erv1p	kcat	Km	kcat/Km
		s−1	mm	m−1s−1
10 mm DTT	WT	1.3 ± 0.1	57 ± 4	2.3 ± 0.2 × 104
	C30S/C33S	1.5 ± 0.1	62 ± 5	2.4 ± 0.2 × 104
	C130S/C133S	<0.1
	C159S/C176S	0.8 ± 0.1	87 ± 8	9.2 ± 0.2 × 103
3.5 mm TCEP	WT	1.1 ± 0.1	27 ± 3	4.1 ± 0.3 × 104
	C30S/C33S	<0.1
	C130S/C133S	<0.05
	C159S/C176S	0.7 ± 0.1	18 ± 2	3.9 ± 0.3 × 104