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. 2009 Aug 24;284(42):28845–28855. doi: 10.1074/jbc.M109.042150

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — PAR1 regulates MUC18 expression in a PAFR-dependent manner. A, PAR1 silencing (shPAR1) down-regulates PAFR expression in A375SM and C8161-c9 metastatic melanoma cells as compared with cells transfected with the control non-targeting shRNA (shNT) (Western blot). B, PAR1 silencing reduces intracellular PAF concentrations in A375SM and C8161-c9 cells, as demonstrated by scintillation proximity assay. Data are mean ± S.D. from three experiments performed in triplicates. *, p < 0.1; **, p < 0.01. C, silencing PAFR expression with small interfering (si)RNA (siPAFR) significantly decreased MUC18 protein expression in A375SM and C8161-c9 melanoma cells as compared with parental cells, cells transfected with Lipofectamine 2000 (Lipofect), or cells transfected with the control non-targeting siRNA (siNT). D, MUC18 expression is rescued in shPAR1 cells transfected with PAFR as compared with shPAR1 cells transfected with control empty pcDNA3.1 vector, suggesting that PAFR mediates MUC18 expression downstream of PAR1.