FIGURE 2.

Extracellular EGF-AB does not affect endogenous PCSK9 effect on LDLR. a and b, HepG2 cells were preincubated for either 4 h (a) or 24 h (b) with 0.01, 0.1, 0.3, 1, 3, and 10 μm EGF-AB peptide together with 0, 67, 135 or 200 nm pure human PCSK9. Then, 5 μg/ml diI-LDL was added, and the incubation continued for another 2 h. Cells were washed and analyzed for fluorescence incorporation. Normalized data are presented. d, Western blot analyses of HepG2 cells incubated for 24 h with 0, 1, 5, and 12.5 μm EGF-AB are shown. These data are representative of three independent experiments.