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. 2009 Jul 27;284(42):28856–28864. doi: 10.1074/jbc.M109.037085

FIGURE 3.

FIGURE 3.

Secreted PCSK9 does not reduce LDLR levels in PCSK9 KO hepatocytes. Primary hepatocytes of Pcsk9−/− (−/−) mice were incubated for 24 h in duplicate with either 100 nm PCSK9 or with 48-h conditioned media from PCSK9 KO or WT hepatocytes (from two mice, WT1 and WT2). Note the lower LDLR levels in Pcsk9+/+ (+/+) hepatocytes, the source of WT media, versus (−/−) hepatocytes. Western blot analyses of LDLR, human PCSK9, and β-actin are shown. Note the level of cell-associated human PCSK9 (hPCSK9) after the 100 nm incubation using a human PCSK9 antibody that does not recognize mouse PCSK9 on Western blots (20). Similar data were obtained in two separate experiments.