FIGURE 3.

HNF1 binding site is critical for PCSK9 transcription and BBR-mediated suppression. A, sequence comparison of proximal regions of PCSK9 promoter of human, mouse, and rat. Gray-highlighted letters indicate divergent nucleotides among the three promoter sequences. B and C, summarized results (means ± S.D.) of eight independent transfections and treatments as described in Fig. 2. In B, the normalized basal luciferase activity of each vector is expressed as the percent of luciferase activity of the D4 vector. In C, the response of each mutant vector to BBR inhibition was compared with that of the wild-type D4 vector. **, p < 0.01; ***, p < 0.001.