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Peptides synthesized. A, sequences of the 3 Skp1 peptide isoforms synthesized, and the corresponding Endo-Lys C-generated peptide from Skp1. B, reverse-phase (C18) HPLC analysis of GlcNAc-O-peptide [1] in an ascending gradient of acetonitrile in 0.1% trifluoroacetic acid, monitored at A215. C, MALDI-TOF-MS analysis of GlcNAc-O-peptide [1].
