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Skp1 α-galactosylation is rescued by overexpressed AgTA-N. A, MALDI-TOF-MS analysis of Skp1 purified from AgtA-N/agtA− cells. Note that Skp1 consists of two genetic isoforms (12, 18), which differ by a Ser or Ala at codon 39 (difference of 16 Da) which broadens the peak. B, similar analysis of glycopeptide purified by RP-HPLC from endo-Lys C-digested Skp1.
