FIGURE 3.

Iwr1p activates transcription when artificially bound to a yeast promoter. The full-length Iwr1p fused to either the LexA DNA binding domain (LexA-IWR1) or LexA alone (LexA) were expressed in the TAT-7 strain. Transcriptional activation was detected by growth on synthetic complete media lacking histidine and containing the indicated amounts of 3-aminotriazole (AT) after 3 days at 30 °C (A), or the expression of a P_GAL1-lacZ reporter gene carrying upstream LexA binding sites (B). Error bars indicate the S. D. of three independent experiments.