A, baker's yeast GR is inactivated by AF: time courses obtained at concentrations of 1 μm (squares), 4 μm (closed circles), 10 μm (open circles), and 50 μm (open triangles). B, effect of BzSe on baker's yeast GR inactivation by AF. Time course in the presence of 4 μm AF (circles) or 4 μm AF plus 2 μm BzSe (triangles). GR exposed to a mixture in which AF 4 μm and BzSe 2 μm were preincubated for 2 h before the assay (pentagons). C, inactivation by AF of truncated SmTGR and wild type SmTGR, and the effect of BzSe. Time courses of truncated SmTGR in the presence of 8 μm AF (circles) or 8 μm AF plus 3 μm BzSe (triangles). Truncated SmTGR exposed to a mixture in which AF 8 μm and BzSe 3 μm were preincubated for 2 h before the assay (pentagons); time course of wild type SmTGR 20 nm incubated with 50 nm AF (open circles).