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. 2009 Aug 28;284(42):28986–28994. doi: 10.1074/jbc.M109.050872

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — CAIN disrupts FLAG-mGluR1a/Gα_q/11 protein complexes. A, representative immunoblot (IB) showing the co-immunoprecipitation of endogenous Gα_q/11 and HA-CAIN with FLAG-mGluR1a from HEK 293 cells. HEK 293 cells were transfected with 3 μg of pcDNA3 plasmid cDNA encoding FLAG-mGluR1a and 3 μg of either empty pcDNA3 plasmid cDNA or pcDNA3 plasmid cDNA encoding HA-CAIN. FLAG-mGluR1a was immunoprecipitated from 500–1000 μg of protein lysate. Also shown is the expression of endogenous Gα_q/11, HA-CAIN, and FLAG-mGluR1a from 25 μg of the corresponding HEK 293 cell lysates. B, densitometric analysis of blots showing the means ± S.E. of four independent experiments. Asterisks indicate significant differences compared with the control (p < 0.05).