FIGURE 3.

A, indicated recombinant Abl proteins were incubated with Sepharose-immobilized GNF-2 or Me-GNF-2 (top), and the bound proteins were visualized by silver staining (bottom). B, the SH3 and/or SH2 domains in Abl are required for GNF-2 to exert its activity. Indicated recombinant Abl proteins were incubated with either DMSO or 10 μm of GNF-2, and then its tyrosine kinase activity was monitored by an ELISA-based assay. Data are expressed as a percentage of DMSO controls (mean ± S.D.; n = 3).