Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 24;284(42):29015–29023. doi: 10.1074/jbc.M109.042820

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — APC S1360A mutation causes off-centered mitotic spindles. A, HeLa cells were transfected with either Myc-tagged APC wild type (WT) or S1360A mutant (Mut), or mock control (Con), and harvested 48 h after transfection. Lysates were immunoblotted for the Myc-tagged APC (myc-APC), which ran just ahead of the 250-kDa marker, and α-tubulin (α-tub) as a loading control. The Myc tag antibody detected a nonspecific band that ran just ahead of the Myc-APC band (the weak band detected in the control transfection lane). B, similarly transfected HeLa cells were fixed 48 h after transfection and stained for the Myc tag to identify overexpressing cells, α-tubulin for microtubules (MT) and DNA. C, quantitation of the percentage of off-centered spindles in APC-overexpressing cells. These data represent >1,000 Myc-tagged APC-expressing cells in three independent experiments.