FIGURE 3.
Determination of the 16-dalton increase by high resolution MS. A and B, high resolution MS spectra of the 827 and 819 m/z peaks (A) as well as the 1141 and 1133 m/z peaks (B) obtained with an Orbitrap mass spectrometer. C, MS/MS spectra of the 827, 1141, 819, and 1133 m/z peaks. D, 155.0808 m/z and 171.0762 m/z fragments exclusively identified as C7H11O2N2 and C7H11O3N2 with 5 ppm mass tolerance.