FIGURE 5.

LAPSER1 regulates the nuclear export of β-catenin after NMDA stimulation in neurons. A, LAPSER-RNAi transfected neurons were nearly completely depleted of endogenous LAPSER1 protein as revealed by immunostaining. B, after 1 h of NMDA application the nuclear import of β-catenin was not altered in cells transfected with either the LAPSER1 RNAi or empty vector construct (Ctrl). C, after 4 h, however, a significant percentage of LAPSER1 RNAi transfected neurons still displayed a prominent β-catenin localization within the nuclear compartment pointing toward the important role of LAPSER1 as a regulator of β-catenin shuttle between the neuronal cytoplasm and nucleus especially with respect to nuclear export. Statistical analysis was performed on the basis of 15 neurons at each time point from three independent experiments applying the Student's t test (*, p < 0.05). DAPI, 4′,6-diamidino-2-phenylindole.