FIGURE 1.

Blom7 is a novel alternatively spliced protein. A, Blom7 isoforms as identified by RT-PCR and sequencing; arrows indicate primers for RT-PCRs. C, RT-PCR using two different primer sets to confirm simultaneous expression of different variants in three different cell lines. Whereas primer pair 1 (P1) shows the expected two bands, P2 shows three bands suggesting additional not yet identified splicing variants. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. B, multiple tissue Northern blots confirms ubiquitous expression of splicing variants in different tissues. D, protein sequence analysis suggests the presence of two KH domains (KH1 and -2), low complexity regions (LCR), and an alternative isoform-specific C terminus.