FIGURE 6.

Blom7α is functionally involved in pre-mRNA splicing. A, addition of recombinant GST-Blom7α enhances in vitro splicing reactions as compared with standard splicing reactions or after addition of GST alone as control. B, this enhancement is dose-dependent, because increasing amounts of GST-Blom7α increase the ratio of lariat to pre-mRNA band as well as the joined exons. Asterisks indicate additional bands specific for Blom7α addition. C, lariat to pre-mRNA ratios were calculated from three independent experiments adding increasing amounts of recombinant Blom7α; untreated nuclear extracts as well as addition of GST were used as controls. NE, nuclear extract.